High-Throughput Fragment Screening by Affinity LC-MS

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

High-throughput identification of IMCD proteins using LC-MS/MS.

The inner medullary collecting duct (IMCD) is an important site of vasopressin-regulated water and urea transport. Here we have used protein mass spectrometry to investigate the proteome of the IMCD cell and how it is altered in response to long-term vasopressin administration in rats. IMCDs were isolated from inner medullas of rats, and IMCD proteins were identified by liquid chromatography/ta...

متن کامل

High-throughput toxicological analysis of Methamphetamine, MDA and MDMA from human plasma by LC-MS/MS

A new high-throughput liquid chromatography coupled with mass spectrometry (LC-MS/MS) method for the quantification of methamphetamine (MA), methylendioximeth-amphetamine (MDMA) and methylendioxiamphetamine (MDA) in human plasma was developed and validated. The separation was performed on a Zorbax SB-C18 column under isocratic conditions using a 15:85 (v/v) mixture of methanol and 0.1% (v/v) fo...

متن کامل

High-throughput analysis of protein/peptide complexes by immunoprecipitation and automated LC-MS/MS.

BACKGROUND The identification of interacting proteins within protein complexes is key to understanding the transduction and regulation of cell signaling pathways, and is also a useful tool for identifying novel disease markers. Immunoprecipitation of protein complexes followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been used to identify targets that bind to a protein o...

متن کامل

High Throughput Quantification of Quinidine in Human Plasma by Lc/ms/ms for Therapeutic Drug Monitoring

A simple, rapid and sensitive HPLC method for the quantification of quinidine in human plasma has been developed and validated. Quinidine was separated on a C18 column under isocratic conditions using a mobile phase of 85:15 (v/v) 0.2% formic acid and acetonitrile. In these chromatographic conditions, the retention time of quinidine was 1.2 min and the overall time of one analysis was 1.6 min. ...

متن کامل

High-Throughput Quantitative LC–MS- MS Assays by On-Line Extraction Using a Monolithic Support

Please direct correspondence to Raymond Xu at [email protected] oday, due to its inherent specificity and sensitivity, the most prevalent bioanalytical technique in drug development is liquid chromatography tandem mass spectrometry (LC–MS-MS). This technique is regarded widely as the preferred tool for quantitative determination of drug and metabolites from biological matrices including pla...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Biomolecular Screening

سال: 2012

ISSN: 1087-0571,1552-454X

DOI: 10.1177/1087057112459271